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. 2019 Apr 26;91(10):6577–6584. doi: 10.1021/acs.analchem.9b00244

Figure 1.

Figure 1

In vivo FPOP workflow. Worms are grown to the fourth larvae stage (L4) on nematode growth media plates. For IV-FPOP, worms are flowed through a 250 μm fused silica capillary in the presence of H2O2, and radicals are generated using a 248 nm wavelength excimer laser. Immediately after irradiation, excess H2O2 and radicals are quenched, worms are lysed, the protein extract is digested and prepared for mass spectrometry analysis, and the extent of FPOP modifications is calculated for proteins of interest.