Figure 5.

Quantitative analysis with HiFRe. a) Counting relative molecular abundance for two sequences present in mixtures at different ratios. A linear fit to y = mx + b yielded m = 0.97 ± 0.04 and b = 0.02 ± 0.02 with R² = 0.993. This strong linear relationship results in a limit of detection of 3.3 ± 2.1%. b) Discrimination and quantitation of SNVs in short DNA sequences. Two sequences differing by three SNVs were mixed together in different ratios, and the plot shows the output ratios recovered after HiFRe analysis. Green bars represent the fraction of the original sequence and yellow bars show the sequence with three SNVs. In both panels, the error bars represent the standard deviation for the mean of two multiplexed sequencing runs.