Fig. 5.

Tamoxifen inhibits the growth and metastasis of prostate tumors in vivo. a, Schematic illustration of the experimental strategy. b, LNCaP-abl-Green cells were implanted into nude mice, with 9 castrated mice in each group. Tumor growth and metastasis were detected with an IVIS system. The data are presented as the mean ± SD. c, Changes in body weight over time (n = 3). d and e, Three primary tumors were selected randomly to be photographed with a digital camera (d), and the volume and weight of the primary tumors were measured (e). The scatter dot plot is presented as the median. *P < 0.05; **P < 0.01 vs. Vehicle+PBS. f, IF analysis of CD49f and ERα or NOTCH1 co-expression in prostate primary tumors tissues of the E2 group. g, Western blot analysis of the indicated proteins in prostate primary tumors tissues (n = 3). h, Number of surviving and metastatic mice injected with LNCaP-abl-Green cells in different groups. Abbreviation: TAM: tamoxifen