Table 6.

List of genes specifically only present in plasmid sequences of at least three L. brevis strains characterized as beer-spoilers

COG category and protein function	L. brevis beer-spoiler strains
	TMW1.2108	TMW1.2111	TMW1.2112	TMW1.2113	UCCLB95	UCCLBBS124	UCCLBBS449
	Defence mechanisms
Membrane protein HorC	+	+	+	–	+	+	+
	Cell wall biogenesis
Lipopolysaccharide biosynthesis glycosyltransferase	+	+	+	+	–	+	+
Lipopolysaccharide biosynthesis glycosyltransferase	+	+	+	+	–	+	+
	Lipid transport and metabolism
Phospholipid-glycerol acyltransferase	+	+	+	+	–	+	+
1-acyl-sn-glycerol-3-phosphate acyltransferase	+	+	+	+	–	+	+
Fatty acid-binding protein DegV	–	–	+	+	–	+	+
	Carbohydrate transport and metabolism
Glycosyl transferase family 2	+	+	+	–	–	+	–
Enolase	+	+	–	–	–	–	+
MFS transporter	+	+	+	+	–	–	–
	Transcription
Sigma-70 region 4 family protein	+	+	–	–	–	–	+
Transcriptional regulator TetR family	+	+	+	–	–	+	+
	Nucleotide transport and metabolism
Cytosine deaminase	+	+	–	+	–	+	+
	Inorganic ion transport and metabolism
CrcB-like protein	+	+	–	–	–	–	+
	Replication, recombination and repair
Cytosine-specific methyltransferase	+	+	–	–	–	+	–
Initiator RepB protein	+	+	–	–	–	–	+
	Function unknown
Hypothetical protein	+	+	–	–	–	–	+
Hypothetical protein	+	+	+	+	–	–	+
Hypothetical protein	+	+	+	+	–	–	–
Hypothetical protein	+	+	–	–	–	–	+
Hypothetical protein	+	+	–	–	–	+	–
Hypothetical protein	+	–	+	–	–	+	–
PemK family protein	+	+	–	–	–	+	–
	Mobilome
Transposase	+	+	–	–	–	+
Mobilization protein	+	+	–	–	–	–	+
Mobilization protein	+	+	–	–	–	–	+

+: gene present, −: gene absent