Fig. 7.

Triptolide disrupts the interaction between TBP and Brf1. a RKO cells expressing FLAG-TBP, Myc-Brf1 were immunoprecipitated with FLAG antibody. The immunoprecipitates were detected with the indicated antibodies. b RKO cells expressing FLAG-Brf1 were immunoprecipitated with FLAG antibody. The immunoprecipitates were detected with the indicated antibodies. c RKO cells expressing FLAG-GFP were immunoprecipitated with FLAG antibody. The immunoprecipitates were detected with the indicated antibodies. d RKO cells expressing the indicated proteins were excited at the wavelength of 458 nm and emission signal between 460 nm to 600 nm was detected at the 10 nm interval. e RKO cells expressing the indicated proteins were treated with 40 nM triptolide for 0.5 h. Cells were excited at the wavelength of 458 nm and YFP (470-500 nm)/CFP (520-550 nm) ratio was calculated