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Additional file 6: Video 5. GNb-FKBP rapidly sequesters endogenously tagged GFP-IP3R1 at the OMM. TIRFM images show HeLa cells with endogenously GFP-tagged IP3R1 and transiently expressing TOM70-mCh-FRB and GNb-FKBP, and then treated with rapamycin (100 nM, added at 60 s). GFP-IP3R1 is rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. 10f and g. (MP4 2601 kb)
