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Additional file 8: Video 7. RNb-FKBP recruits a PM protein to the OMM in response to rapamycin. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and the PM protein, β2AR-mCh, and then exposed to rapamycin (100 nM, added at 60 s). There is a rapid translocation of β2AR-mCh to the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. 12b–e. (MP4 2141 kb)
