FIG. 1.

Disruption of the interaction between nuclear receptor co-repressor-1 (NcoR1) and thyroid hormone receptor alpha (TRα) ameliorates the skeletal phenotype in Thra1^PV/+ mice. (A) X-ray microradiography images of femurs from male wild-type (WT), Thra1^PV/+, and Thra1^PV/+NCoR1^ΔID/ΔID mice at 14 weeks of age. Gray-scale images and pseudocolored images in which low bone mineral content (BMC) is indicated in green and high BMC in pink. Cumulative frequency histogram of relative BMC (n = 5–8 per group). ***p < 0.001 vs. WT; Kolmogorov–Smirnov test. Graphs show lengths of femurs, humeri, and vertebrae. Data are shown as the mean ± standard error of the mean (SEM; n = 4–8 per group). **p < 0.01 and ***p < 0.001; analysis of variance (ANOVA) followed by Tukey's post hoc test. (B) Micro computed tomography (micro-CT) images of mid-diaphysis cortical bone. Graphs showing cortical thickness (Ct.Th), cortical bone mineral density (BMD), and cortical porosity (Ct.Po). Data are shown as the mean ± SEM (n = 5–8 per group apart from Ct.Po, n = 3). *p < 0.05 and **p < 0.01; ANOVA followed by Tukey's post hoc test. (C) Micro-CT images of distal femur trabecular bone. Graphs showing trabecular bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular spacing (Tb.Sp). Data are shown as the mean ± SEM (n = 5–8 per group). **p < 0.01, ***p < 0.001, and ****p < 0.0001; ANOVA followed by Tukey's post hoc test. (D) Representative load displacement curves for humerus three-point bend testing. Yield load, maximum load, fracture load, and stiffness. Data are the mean ± SEM (n = 5–8 per group). *p < 0.05, **p < 0.01, and ***p < 0.001; ANOVA followed by Tukey's post hoc test. Scale bars in (A), (B) and (C) = 1 mm. Data from the same group of untreated WT mice are included in Figures 1–4 to facilitate comparison across groups. Color images are available online.