FIG. 3.
Treatment with suberoylanilide hydroxamic acid (SAHA) has no effect on bone mass, mineralization, or strength in WT or Thra1PV/+ mice. (A) X-ray microradiography images of femurs from male WT, SAHA-treated WT (WT SAHA), Thra1PV/+, and SAHA-treated Thra1PV/+ (Thra1PV/+ SAHA) mice at 14 weeks of age. Gray-scale images and pseudocolored images in which low BMC is indicated in green and high BMC in pink. Cumulative frequency histogram of relative BMC (n = 5–8 per group). **p < 0.01 SAHA treated vs. untreated; Kolmogorov–Smirnov test. Graphs show lengths of femurs, humeri, and vertebrae. Data are shown as the mean ± SEM (n = 5–8 per group), treated vs. untreated; Student's t-test. (B) Micro-CT images of mid-diaphysis cortical bone. Graphs showing Ct.Th, cortical BMD, and Ct.Po. Data are shown as the mean ± SEM (n = 5–8 per group apart from Ct.Po, n = 3). (C) Micro-CT images of distal femur trabecular bone. Graphs showing BV/TV, Tb.N, Tb.Th, and Tb.Sp. Data are shown as the mean ± SEM (n = 5–8 per group). (D) Representative load displacement curves for humerus three-point bend testing. Yield load, maximum load, fracture load, and stiffness. Data are the mean ± standard error of the mean (n = 5–8 per group); treated vs. untreated. Scale bars in (A), (B) and (C) = 1 mm. Data from the same group of untreated WT mice are included in Figures 1–4 to facilitate comparison across groups. Color images are available online.