FIG. 4.

Treatment with SAHA has no effect on bone mass, mineralization, or strength in NCoR1^ΔID/ΔID or Thra1^PV/+NCoR1^ΔID/ΔID mice. (A) X-ray microradiography images of femurs from male WT, NCoR1^ΔID/ΔID, SAHA-treated NCoR1^ΔID/ΔID (NCoR1^ΔID/ΔID SAHA), Thra1^PV/+NCoR1^ΔID/ΔID, and SAHA-treated Thra1^PV/+NCoR1^ΔID/ΔID (Thra1^PV/+NCoR1^ΔID/ΔID SAHA) mice at 14 weeks of age. Gray-scale images and pseudocolored images in which low BMC is indicated in green and high BMC in pink. Cumulative frequency histogram of relative BMC (n = 6–8 per group). SAHA treated vs. untreated; Kolmogorov–Smirnov test. Graphs show femur, humerus and vertebral lengths. Data are shown as the mean ± SEM (n = 6–8 per group); treated vs. untreated. **p < 0.01; Student's t-test. (B) Micro-CT images of mid-diaphysis cortical bone. Graphs showing Ct.Th, cortical BMD, and Ct.Po. Data are shown as the mean ± SEM (n = 6–8 per group apart from Ct.Po, n = 3). (C) Micro-CT images of distal femur trabecular bone. Graphs showing BV/TV, Tb.N, Tb.Th, and Tb.Sp. Data are shown as the mean ± SEM (n = 6–8 per group). (D) Representative load displacement curves for humerus three-point bend testing. Yield load, maximum load, fracture load, and stiffness. Data are the mean ± SEM (n = 6–8 per group); treated vs. untreated. Scale bars in (A), (B) and (C) = 1 mm. Data from the same group of untreated WT mice are included in Figures 1–4 to facilitate comparison across groups. Color images are available online.