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. 2019 May 17;10:1003. doi: 10.3389/fimmu.2019.01003

Figure 2.

Figure 2

NETs induced cytotoxicity in BMECs. (A)The cytotoxicity of BMECs was determined after treatment with NET (484 ng/mL), NET (484 ng/mL) + DNase I or DNase I for 16 h. Lysis provided by LDH Cytotoxicity Assay Kits was used as positive controls. (B) The viability of BMECs was detected by Cell Counting Kit-8. Briefly, BMECs were treated with NET (342 ng/mL), NET (342 ng/mL) + DNase I (1.5 mg/mL), NET (342 ng/mL) + DNase I (3 mg/mL), or NET (342 ng/mL) for 16 h. After washed for three times, the samples were treated with 10 μL CCK-8, incubated for 2 h and examined microplate reader at wavelength at 450 nm. Date are presented as mean ± SEM (n = 5). P < 0.05 were considered significant (*P < 0.05, ***P < 0.001, and “ns” means not significant).