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. 2019 May 17;10:1003. doi: 10.3389/fimmu.2019.01003

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Copyright © 2019 Wei, Wang, Wang, Wang, Liu, Han, Fu and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analyses of histone caused BMECs apoptosis, necrosis, and pyroptosis. BMECs were incubated with stimulated histone for 16 h. Then, the BMECs were stained FLUOS-Annexin V/PI according to the manufacturer's protocols and were examined by Flow cytometry. The results represent that Annexin V⁻/PI⁻ (LL) and Annexin V⁻/PI⁺ (Pyroptosis, UL), Annexin V⁺/PI⁻ (Apoptosis, LR), and Annexin V⁺/PI⁺ (Necrosis, UR). (A) Controls. (B) BMECs treated with histone (50 μg/mL). (C). BMECs treated with histone (100 μg/mL). (D) BMECs treated with histone (200 μg/mL). Histone caused BMECs necrosis (5.02%), pyroptosis (3.28%), and apoptosis (1.10%) in a dose-dependent. Three independent experiments were carried out by flow cytometry analyses.