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Histone induce BMECs pyroptosis is dependent on activations of caspase 1, caspase 3 and NLRP3. BMECs were incubated with stimulated histone for 16 h, and the activities of these proteins were determined by western blotting. Date are presented as mean ± SEM (n = 5). P-values of < 0.05 were considered significant (**P < 0.01, ***P < 0.001, and “ns” means not significant).
