Figure 5.

Cyclin D1 expression after OTUD6B-AS1 knockdown in dermal fibroblasts (Fb) or human pulmonary artery smooth muscle cells (HPASMC). HC dermal Fb or HPASMC were transfected with locked nucleic acid antisense oligonucleotide (ASO) negative control or ASO targeting OTUD6B-AS1. (A) mRNA level of cyclin D1 (CCND1) expression was measured 48 h after transfection (n = 6) and Cyclin D1 protein was measured 72 h after transfection (n = 6) in dermal Fb by Western blot. (B) mRNA level of cyclin D1 (CCND1) expression (n = 8, biological replicates) and Cyclin D1 protein (n = 4, biological replicates) was measured 48 h after transfection in HPASMC by Western blot. mRNA expression was normalized by GAPDH and RPLP0 and protein expression was normalized by GAPDH. Data are shown as single values and mean. Statistical analysis was performed by paired t-test. k.d, knockdown.