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. 2019 May 17;10:1100. doi: 10.3389/fimmu.2019.01100

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Copyright © 2019 Takata, Pachera, Frank-Bertoncelj, Kozlova, Jüngel, Whitfield, Assassi, Calcagni, Vries-Bouwstra, Huizinga, Kurreeman, Kania and Distler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OTUD6B expression after OTUD6B-AS1 knockdown in dermal fibroblasts (Fb, A) or human pulmonary artery smooth muscle cells (HPASMC, B). HC dermal Fb or HPASMC were transfected with locked nucleic acid antisense oligonucleotide (ASO) negative control or with ASO targeting OTUD6B-AS1. (A) mRNA of total OTUD6B was measured 72 h after transfection (n = 5). OTUD6B protein was measured 72 h after transfection (n = 6) in dermal Fb by Western blot. (B) mRNA of total OTUD6B was measured 48 h after transfection (n = 4, biological replicates). OTUD6B protein expression was measured 48 h after transfection by Western blot (n = 4, biological replicates) in HPASMC. mRNA expression was normalized by GAPDH and RPLP0 and protein expression was normalized by GAPDH. (C) The subcellular localization of OTUD6B-AS1 in SSc dermal Fb was analyzed by qPCR (n = 4). Expression levels were normalized by GAPDH and RPLP0. Data are shown as single values and mean. Statistical analysis was performed by paired t-test. k.d, knockdown.