Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 17;13:484. doi: 10.3389/fnins.2019.00484

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Zhu, Xiang, Li, Lin, Hu, Zhang, Meng, Liu, Chen, Shu, Zhang, Zhang, Si and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Specific knockout of the CB1 receptor on GABAergic neurons localized in the vlPAG. (A) rAAV-mDlx-CRE-WPRE-pA was injected into the right side of the vlPAG of mCnr1flox/flox mice. (a) GABA-immunoreactive cellsin the vlPAG (green). (b) CB1-immunoreactive cells (red). (c) DAPI nuclear staining (blue). (d) GABA-immunoreactive and DAPI nuclear staining colocalization fluorescence (purple); Yellow arrows show CB1 receptors not knocked out on GABAergic neurons. White arrows show CB1 receptors not knocked out on other large diameter neurons. Scale bar, 100 μm. (B) rAAV-CaMKII-CRE-WPRE-pA was injected into the right side of the vlPAG of mCnr1flox/flox mice. (a) GLU-immunoreactive cells (green). (b) CB1-immunoreactive cells in the vlPAG (red). (c) DAPI nuclear staining (blue). (d) GLU-immunoreactive and DAPI nuclear staining colocalization fluorescence (purple); White arrows show CB1 receptors not knocked out on glutamatergic neurons. Scale bar, 100 μm. (C) Summary data show that the percentage of CB1-immunoreactive cells was knocked out in the area of GABA-immunoreactive cells. Blue sector, GABA-immunoreactive cells; red sector, CB1-immunoreactive cells were knocked out; green sector, residual cells. (D) Summary data shows the percentage of CB1-immunoreactive cells knocked out in the area of glutamatergic-immunoreactive cells. Pink sector, glutamatergic-immunoreactive cells; red sector, CB1-immunoreactive cells been knocked out; green sector, residual cells. Data are expressed as the means ± SEM (n = 3 mice in each group).