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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Dec 17;127:116–124. doi: 10.1016/j.yjmcc.2018.12.006

Fig. 4. Knockdown of Atg2, Atg9 or Atg18 results in reduced mitochondrial density and abnormal mitochondrial structure in IFM.

Fig. 4.

Confocal microscopy was performed for IFMs in Atg knockdown and WT control flies at 40 days of age along with Parkin knockdown flies as positive control. A) Representative images of MitoTimer signals (merged image of both GFP and DsRed channels) in IFM. Knockdown of Atg2 show increased numbers of fragmented (arrow head) and hollow mitochondria (arrows), similar to Parkin knockdown flies. Scale bar =10 μm; and B) Quantification of mitochondrial density in IFM (n = 14. *, ** and *** denote p < 0.05, p < 0.01 and p < 0.001, respectively.