Table 1.
HBoV1 capsid helper plasmids
| Helper name | Expression of HBoV1 proteins | Mutations of (pA)p sites | Cap codon-optimized | Other helper functions |
|---|---|---|---|---|
| pHBoV1KUm630 | NS1–4, NP1, VP1–3 | No | No | No |
| pCMVNSCap | NS1–4, NP1, VP1–3 | No | No | No |
| pCMVNS*Cap | NS3–4, NP1, VP1–3 | No | No | No |
| pCMVNP1Cap | NP1, VP1–3 | No | No | No |
| pCMVR7cDNAm(pA)p | VP1–3 | Yes | No | No |
| pCMVNS*Cap-Rep2 | NS3–4, NP1, VP1–3 | No | No | AAV2 Rep |
| pCMVint-NP1Cap-Rep2 | NP1, VP1–3 | No | No | AAV2 Rep |
| pCMVint-NP1Cap*-Rep2 | NP1 | No | No | AAV2 Rep |
| pcDNAoptVP | VP1–3 | Yes | Yes | No |
| pcDNAoptVP1(ATG) | VP1–3 | Yes | Yes | No |
| pCMVoptVP1-Rep2 | VP1 | Yes | Yes | AAV2 Rep |
| pAd4.1-CMVoptVP2/3 | VP2–3 | Yes | Yes | Adv VAI, E2a, E4 ORF6 |
HBoV1, human bocavirus 1; AAV2, adeno-associated virus 2; Adv, adenovirus.