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. 2019 May 13;28(10):659–673. doi: 10.1089/scd.2019.0030

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© Sherri M. Biendarra-Tiegs et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — Single-cell RNA-seq analysis in hiPSC-derived cardiac differentiation clearly separates days 12 and 40 cells with distinct electrophysiological properties. (A) Schematic of experimental design. hiPSC-CMs were analyzed at days 12 and 40 of differentiation via single-cell RNA-seq and ArcLight. (B) Unsupervised hierarchical clustering on the entire transcriptomic profiles of all cells (D12: n = 42 cells; D40: n = 43 cells). (C) Principal component analysis compiled from the entire transcriptomic profiles of all cells. (D) Representative inverted fluorescent traces for ventricular-like morphology (from D12) and atrial-like morphology (from D40). (E) Percentage of total cells at D12 or D40 with each AP morphology classification. “Indeterminate” cells had either insufficiently mature APs or demonstrated a mixture of atrial- and ventricular-like APs. (F) AP amplitude (left), V_max (middle), and APD₉₀/APD₅₀ (right) evaluated from D12 and D40 populations before submission for sequencing. P values were calculated by either a Student's t-test or Mann–Whitney U test. Data are reported as median ± interquartile range. hiPSC, human induced pluripotent stem cell.