FIG. 5.

Several ion channel regulators exhibit increased expression with electrophysiological maturation of hiPSC-CMs. (A) Heatmap representation of expression patterns for representative electrophysiology- and calcium handling-associated genes in cardiomyocytes. Genes with significantly different expressions (log₂FC >2 and P < 0.05) between days 12 and 40 are marked with an asterisk. (B–D) Relative expression (log₂FC) of SLMAP (B), FGF12 (C), or FHL1 (D) D40 gene expression for both single-cell RNA-seq (cardiomyocytes only) and qRT-PCR, compared with D12 samples. Error bars represents SEM. qRT-PCR data were obtained from one to three clones each derived from three unrelated individuals. Student's t-test analysis was used to calculate P values associated with the qRT-PCR data. FC, fold change; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; SEM, standard error of the mean.