Table 1. Bacteria strains and plasmids used in this study.
| Strains or plasmids | Characteristics | Source |
|---|---|---|
| Strain | ||
| R. erythropolis KB1 | Wild-type | Laboratory collection |
| E. coli DH5α | TIANGEN, China | |
| E. coli BL21 (DE3) | TIANGEN, China | |
| BL21-pET-32a (+)-rpf-1 | Ampr, BL21containing pET-32a (+)-rpf-1 | This work |
| Plasmids | ||
| pET-32a (+) vector | Ampr, 5.9 kb, high-copy-number cloning vector | Novagen |
| pUCm-T-rpf-1 | Ampr, pUCm-T with an 564 bp fragment containing rpf-1 | This work |
| pET-32a (+)-rpf-1 | Ampr, pET-32a (+) with an 564 bp fragment containing rpf-1 | This work |