. 2019 May 21;7:e6951. doi: 10.7717/peerj.6951

Table 2. The conserved amino acid resides for substitution and the related oligo nucleotide sequences.

Mutant proteins	Mutations	Oligo nucleotides (5′–3′)
E51A	Glu⁵¹→Ala	GTCTCGCCCAGTGTGCGGCCGGCGGAAACTG
D45A+E51K	Asp⁴⁵→Ala	CCGACTCCGACTGGTATCGTCTCGCCCAGTG
D45A+E51K	Glu⁵¹→Lys	GTCTCGCCCAGTGTAAGGCCGGCGGAAACTG
C50G+C114T	Cys⁵⁰→Gly	ATCGTCTCGCCCAGGGTGAGGCCGGCGGAAA
C50G+C114T	Cys¹¹⁴→Thr	GCGCATGGCCGTCCACCTCCTCCAGCCTCGG
T60A	Thr⁶⁰→Ala	ACTGTGCCATCAACGCGGGCAACGGTTACCA
Q69K	Gln⁶⁹→Lys	ACCAGGGCGGACTGAAGTTCTCCCCCAGCAC
T74A	Thr⁷⁴→Ala	AGTTCTCCCCCAGCGCGTGGAACGCACACGG
W75V	Trp⁷⁵→Val	GTTCTCCCCCAGCACGGTGAACGCACACGGCGGCG