Table 3. Effects of metal ions and chemical agents on activities of the purified recombinant Rpf-1 of R. erythropolis KB1.
| Chemical reagents | Final concentrations (mM) | Relative activity (%) | |
|---|---|---|---|
| Control | 100.00 | ||
| Zn2+ | 0.1 | 260.83 ± 12.25 | |
| Mg2+ | 0.1 | 96.90 ± 3.98 | |
| Co2+ | 0.1 | 98.40 ± 2.55 | |
| Ca2+ | 0.1 | 110.14 ± 3.59 | |
| DTT | 0.1 | 98.88 ± 4.21 | |
| PMSF | 0.1 | 109.43 ± 5.07 | |
| EDTA | 0.1 | 99.81 ± 3.22 | |
| EGTA | 0.1 | 98.49 ± 4.14 |
Note:
Purified enzyme was pre-incubated with metal ions and biochemical reagents for 30 min. The enzyme activities of the pre-incubated recombinant protein without reagents were taken as 100%. The activities represent the mean of at least two determinations carried out in duplicates, and the value display with the mean ± SD.