Table 4. The enzymatic activities of the wild-type and mutant Rpfs of R. erythropolis KB1.
| Proteins | Muralytic activity | Protease activity | ||
|---|---|---|---|---|
| Specific activity (u mg−1) | Percent activity retained (%) | Specific activity (u mg−1) | Percent activity retained (%) | |
| Rpf-1 | 1,760 ± 129 | 100.00 | 1,630 ± 310 | 100.00 |
| E51A | 1,570 ± 230 | 88.80 ± 6.60 | 0 | 0 |
| D45A+E51K | 270 ± 30 | 15.60 ± 0.60 | 0 | 0 |
| C50G+C114T | 230 ± 10 | 13.30 ± 0.40 | 0 | 0 |
| T60A | 1,700 ± 100 | 96.70 ± 1.00 | 1,560 ± 100 | 98.30 ± 12.80 |
| Q69K | 240 ± 20 | 13.90 ± 0.30 | 0 | 0 |
| T74A | 1,700 ± 80 | 96.70 ± 2.20 | 0 | 0 |
| W75V | 3,430 ± 420 | 194.40 ± 9.70 | 0 | 0 |
Note:
The activities were determined at three replications and display with the mean ± SD.