Skip to main content
. Author manuscript; available in PMC: 2019 Aug 25.
Published in final edited form as: Nat Cell Biol. 2019 Feb 25;21(3):384–396. doi: 10.1038/s41556-019-0281-x

Fig. 8. Expression of ALS-variant containing UBQLN2 (UBQLN2P497H) impairs lysosomal degradation.

Fig. 8.

(a-d) Western blots for UBQLN2, dUbqn, Phospho-eIF2α (S51), total eIF2α, p62, Atg8, and V100 with fat body protein lysates from third instar larvae of w; Cg-GAL4/UAS-Luciferase, w; Cg-GAL4/UAS-FLAG-dUbqn, w; Cg-GAL4/UAS-UBQLN2WT-HA, and w; Cg-GAL4/UAS-UBQLN2P497H-HA. Two independent experiments were performed with similar results.

(e) Live imaging for LysoTracker (LT) in third instar fat body of w; Cg-GAL4/UAS-Luciferase, w; Cg-GAL4/UAS-FLAG-dUbqn, w; Cg-GAL4/UAS-UBQLN2WT-HA, and w; Cg-GAL4/UAS-UBQLN2P497H-HA. Two independent experiments were performed by n=10 biologically independent samples with similar results. Scale bars, 20 μm.

(f) Ubiquilins regulate autophagic flux through mTOR signaling and lysosomal acidification