Figure 1.

Honokiol inhibits the production and hemolytic activity of staphylococcal Hla. (a) WB analysis of Hla production. Culture supernatants of S. aureus 8325-4 grown in the absence or presence of subinhibitory concentrations of Honokiol were detected with a specific antibody against Hla. (b,c) Hemolysis assays were performed with rabbit red blood cells in PBS. The addition of Honokiol reduced the hemolysis, as indicated by the colour attenuation (b), and the OD absorbance at 543 nm decreased (c). Bars show the mean values of the experiments (n = 3). **Indicates P < 0.01 when compared with the control group.