Table 2. Summary of diagnostic assay results for weaned pig cases.
| Case no. | Site state | Site ID | Age (wk) | Clinical signs | Gross | Histopathology | PCR Cqa | ISHe | ||
|---|---|---|---|---|---|---|---|---|---|---|
| PCV3b | PCV2c | PRRSd | ||||||||
| 1 | IA | K | 3 | Dermatopathy | Palpable purpura | Haired skin: Vasculitis and perivasculitis, fibrinoid and leukocytoclastic with epidermal necrosis | 23.4 | Uf | U | Skin: Adipocytes in panniculus LNg: Rare lymphocytes |
| 2h | IA | L | 3 | Derma-topathy | Palpable purpura | Haired skin: Vasculitis and perivasculitis, fibrinoid and leukocytoclastic with epidermal necrosis Heart, kidney, and mesenteric arteries: Periarteritis and arteritis, lymphohistiocytic and plasmacytic |
26.5 | U | NPi | SIj: Adipocytes of mesentery, mesothelial cells, rare crypt cells, rare enterocytes, rare smooth muscle of tunica muscularis, smooth muscle of arteries LIk: Smooth muscle of arteries Skin: Endothelial cells, epithelium of hair follicle, adipocytes in panniculus, epithelium |
| 3 | IA | M | 5 | Sudden death | DUl | Heart, kidney, mesentery of spleen: Periarteritis and arteritis, lymphohistiocytic and plasmacytic | 25.0 | U | NP | Lung: Low numbers of mesothelial cells of pleura, pneumocytes, smooth muscle of arteries Liver: Not detected Heart: Occasional cardiac myocytes, smooth muscle of arteries, lymphocytes surrounding arteries Kidney: Smooth muscle of artery Spleen: Mesothelial cells of mesentery, smooth muscle of arteries in spleen and mesentery, adipocytes in mesentery, smooth muscle of trabeculae, lymphocytes |
| 4 | IA | M | 8 | Respc | DU | Heart: Lymphohistiocytic myocarditis, multifocal, mild with segmental periarteritis Kidney: Interstitial nephritis, lymphohistiocytic, multifocal, moderate |
25.8 | U | 31.4 | Liver: Hepatocytes in low numbers commonly in clusters Heart: Rare cardiac myocytes |
| 5 | IA | M | 9 | Resp | DU | Kidney: Periarteritis, lymphohistiocytic and plasmacytic | 27.0 | U | U | Spleen: Lymphocytes Lung: Not detected Kidney: Rare renal tubular epithelium; adipocytes surrounding kidney |
| 6 | IA | N | 10 | Sudden death | DU | Heart: Periarteritis, lymphohistiocytic | 28.0 | U | 23.3 | Heart: Smooth muscle of artery |
| 7 | IA | O | 3 | Enteric | Fluid filled LI | Heart: Periarteritis and arteritis, lymphohistiocytic and plasmacytic Large intestine; mesocolon: Periarteritis and arteritis, lymphohistiocytic and plasmacytic |
22.7 | U | U | Heart: Cardiac myocytes and smooth muscle of arteries LI: Scattered smooth muscle cells of the tunica muscularis; lymphocytes of GALT Mesocolon: Smooth muscle of arteries LN: Low numbers of lymphocytes Thymus: Not detected Liver: Rare hepatocytes |
| 8 | MN | P | 6 | Resp | Fibrinous epicarditis | Heart, kidney, and mesocolon: Perivasculitis, lymphocytic Heart: Myocarditis, lymphohistiocytic and fibrinous epicarditis |
23.2 | U | U | Heart: Smooth muscle of arteries; cardiac myocytes LI: Smooth muscle of tunica muscularis; rare colonocytes, adipocytes; smooth muscle of arteries in mesocolon Liver: Rare hepatocytes; smooth muscle of artery |
| 9 | NC | Q | 5 | Resp | DU | Heart and kidney: Periarteritis and arteritis, lymphohistiocytic | 23.6 | U | U | Kidney: Smooth muscle cells of artery and mononuclear inflammatory cells Heart: Rare cardiac myocytes |
| 10 | IL | R | NA | Poor condition | DU | Myocarditis and periarteritis | 19.9 | U | U | Heart: Smooth muscle cells of an artery; rare cardiac myocytes |
| 11 | IL | S | 4 | Resp | DU | A: Myocarditis, lymphocytic Spleen; mesentery: Arteritis, focal B: Myocarditis, periarteritis, arteritis Kidney: Periarteritis and arteritis |
27.2-A 22.1-B |
U | 31.1-B | A: Rare cardiac myocytes and renal tubular cells B: Cardiac myocytes, adipose surrounding kidney, and smooth muscle of arteries in the heart and kidney |
aCq: Cycle quantification value.
bPorcine circovirus 3.
cPorcine circovirus 2.
dPorcine reproductive and respiratory syndrome virus.
eISH: In situ hybridization; tissues in which PCV3 was detected by RNAscope® ISH assay.
fU: Undetected after cycle cut-off.
gLN: Lymph node.
hCase No. 2-5: Pigs originated from the same sow source as site ID K.
iNP: Not performed.
jSI: Small intestine.
kLI: Large intestine.
lDU: Diagnostically unremarkable.
mResp: Respiratory.