Figure 3.

ASYN-CONA, a bead-based assay for α-synuclein aggregation. Upper panel shows a schematic representation of the assay. Lower panel shows example images acquired at different stages of aggregation. In the image on the left, α-synuclein labeled with TMR (green) is attached to the beads, which is observed as rings in fluorescence emission channel for TMR. Cy5-labeled α-synuclein is added to the solution but no bead rings are observed in Cy5 fluorescence emission channel (red), because aggregation has not yet started. In the middle image, upon treatment with an aggregation inducing agent, red rings of Cy5-labeled α-synuclein begin to form around the bead. In the image on the right, over time, α-synuclein aggregates increase in size, leading to an increase in the fluorescence emission ring intensity in the Cy5 channel.