Table 1. Observed Aggregation Rates of α-Synuclein-A91C-Cy5 (500 nM), as a Function of the Amount of α-Synuclein-A91C-HTM on Bead^a.

	Intercept (AU)		Slope (h–1)		Statistics
αS-A91C-HTM	value	std er	value	std er	adj. R2
0 nM	1.82	0.15	9.78	2.09	0.81
500 nM (no EtOH)	1.85	0.34	6.01	1.11	0.85
500 nM	2.19	0.36	326.94	17.56	0.99
250 nM	2.06	0.18	221.31	8.74	0.99
100 nM	2.45	0.07	149.22	2.74	1.00
50 nM	2.31	0.08	67.24	1.68	1.00
25 nM	2.34	0.07	51.07	1.16	1.00

^aResults from the fitting of the mean bead ring intensity in the Cy5 emission channel of data from Figure 4 for the different concentrations of α-synuclein-A91C-HTM incubated with the beads, using y = a + bx as a linear equation. At higher concentrations of α-synuclein-A91C-HTM, the slope of the fit increases, and the adjusted R² decreases.