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. 2019 May 21;10(36):3315–3327.

Figure 3. DUSP6 inhibition alters expression of ERK pathway responsive genes.

Figure 3

OVCAR8 and SKOV3 cells were treated with BCI, carboplatin, BCI+carboplatin, paclitaxel, or BCI+paclitaxel for 24 h and qPCR was performed. (A) BCI opposed the effect of rHE4 on EGR1 levels in OVCAR8 cells, and EGR1 mRNA levels were higher in cells co-treated with BCI and chemotherapeutic drugs than in cells treated with chemotherapy alone. (B) BCI opposed the effect of rHE4 on c-JUN levels in OVCAR8 cells, and c-JUN mRNA levels were lower in cells co-treated with BCI and chemotherapeutic drugs than in cells treated with chemotherapy alone. (C) BCI opposed the effect of rHE4 on EGR1 levels in SKOV3 cells, and EGR1 mRNA levels were higher in cells co-treated with BCI and chemotherapeutic drugs than in cells treated with chemotherapy alone. (D) BCI opposed the effect of rHE4 on c-JUN levels in SKOV3 cells, and c-JUN mRNA levels were lower in cells co-treated with BCI and chemotherapeutic drugs than in cells treated with chemotherapy alone. Error bars represent standard deviation of n≥3 independent experiments. *p<.05; **p<.005; ***p<.0005; ***p<.00005.