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. Author manuscript; available in PMC: 2020 Jun 3.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Apr 23;58(23):7611–7614. doi: 10.1002/anie.201810919

Figure 4.

Figure 4.

Combination of amperometry with TIRF imaging demonstrates that NS510 can resolve catecholamine release from an individual vesicle. Three recordings from three different bovine adrenal chromaffin cells on an Au ring electrode demonstrate punctate changes in NS510 fluorescence synchronous with amperometric spikes resulting from oxidation of catecholamine release from a single vesicle. Circles depict the regions of interest where changes in fluorescence were observed as quantified in Arbitrary Units (AU) in the horizontal lines below the amperometric traces. (Scale bar = 5 µm)