Fig. 3.

Molecular basis for the specific targeting of H4K16ac by MLL4-PHD6. a Superimposed ¹H,¹⁵N heteronuclear single quantum coherence (HSQC) spectra of the linked H4_11–23-G₇-PHD6 construct, wild-type (black) and indicated mutants (green, light blue, and orange), the isolated MLL4-PHD6 domain (blue), isolated H4-bound MLL4-PHD6 (dark yellow), and isolated H4K16ac-bound MLL4-PHD6 (red). b The structure of the H4K16ac-bound MLL4-PHD6 finger. c Representative binding curves used to determine the K_d values for the L1504E mutant by fluorescence spectroscopy (also see Supplementary Fig. 5). Source data are provided as a Source Data file. d Superimposed ¹H,¹⁵N HSQC spectra of MLL4-PHD6 mutants collected upon titration with H4K16ac peptide. Spectra are color coded according to the protein:peptide molar ratio