Fig. 5.

Targeting of MLL4 to active promoters is dependent on H4K16ac mark. Mof^f/f;Cre-ER brown preadipocytes were treated with 4-hydroxytamoxifen and collected for chromatin immunoprecipitation–sequencing (ChIP-Seq) analysis. a ChIP-Seq profiles of MLL4 and H4K16ac on Plin3 and Zhx1 gene loci. b Deletion of Mof decreases MLL4 binding to all sites. c H4K16ac-enriched (H4K16ac⁺) MLL4-binding regions are mainly located on active enhancers and active promoters. d Heat maps of 4591 Mof-dependent H4K16ac⁺ MLL4-binding sites. e Gene ontology analysis of the genes associated with 4591 Mof-dependent H4K16ac⁺ MLL4-binding regions identified in d. f Motif analysis of 4591 Mof-dependent H4K16ac⁺ MLL4-binding regions identified in d