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. 2019 May 24;9:7799. doi: 10.1038/s41598-019-44222-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Terminal sprouting at endplates in contralateral and reinnervated SOL muscles. Confocal maximum projections of endplates after whole mount staining for Schwann cells (S100), axons and nerve terminals (NF/SV2) and AChRs (BTX). Arrows point to terminal sprouts. (A) Contralateral SOL: An example of a NMJ without terminal sprouts in DMSO WT SOL, and two examples of NMJs with two short terminal sprouts each in LD and HD SMA SOL. (B) Denervated SOL: Endplates with more profuse terminal sprouting 7 days after crush denervation in all treatment groups. Scale bars: 20 μm (A). 30 μm (B).