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. 2019 May 24;9:7799. doi: 10.1038/s41598-019-44222-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell marker distribution of terminal sprouts. (A) An example of a NMJ in a LD SMA contralateral SOL with one sprout (arrow) that labeled positively for S100 but negatively for NF/SV2. This was thus a tSC terminal sprout without an associated nerve sprout. Scale bar: 25 μm. (B) Cell marker distribution of terminal sprouts in contralateral SOL. While all sprouts labeled for both S100 and NF/SV2 in DMSO WT SOL, about 20% of sprouts only labeled for S100 in SMA muscles. (C) Cell marker distribution of terminal sprouts in reinnervated SOL. Most sprouts labeled for both S100 and NF/SV2 in all groups. Sprouts scored in contralateral and denervated SOL, respectively: DMSO WT: 10 and 114; LD SMA: 61 and 137; HD SMA: 79 and 180.