Table 2.

Rabbit anti-rPhl p 7 mutant antibodies inhibit patients’ IgE binding to the Phl p 7 wildtype protein.

Patient	rabbit pre-immune serum (OD 405 nm)	rabbit anti-rPhl p 7 mutant serum (OD 405 nm)	% of inhibition
1	1.677 ± 0.012	0.227 ± 0.001	86.5
2	1.130 ± 0.026	0.255 ± 0.001	77.4
5	2.270 ± 0.012	0.943 ± 0.057	58.5
6	0.851 ± 0.028	0.198 ± 0.005	76.7
8	1.470 ± 0.027	0.327 ± 0.007	77.8
9	1.375 ± 0.012	0.220 ± 0.003	84.0
12	0.740 ± 0.009	0.197 ± 0.020	73.4
13	0.458 ± 0.017	0.141 ± 0.000	69.3
N.A.	0.115 ± 0.004	0.158 ± 0.004	0

ELISA plate-bound rPhl p 7 wildtype was preincubated with 1:100 diluted rabbit anti-rPhl p 7 mutant serum or with the preimmune serum and subsequently exposed to the sera from eight Phl p 7-sensitized patients or from a patient sensitized to an unrelated allergen source (N.A.). IgE binding was measured by ELISA and mean OD values at 405 nm and percentage inhibition of IgE binding is displayed.