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. 2019 May 24;9:7811. doi: 10.1038/s41598-019-44204-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stratum corneum ceramide composition of FTMs generated under hypoxia. (a) Level of total lipids in the SC of FTMs generated at indicated oxygen level and NHS. (b) Ion maps as detected by LC-MS of FTMs generated under normoxia or hypoxia and NHS. Twelve CERs_total subclasses are identified. (c) Cumulative amount of quantified CERs_total per mg SC in FTMs and NHS. (d) CER and CER EO subclass profile of FTMs and NHS. Tabular inset provides subclass ratio defined by $\frac{(d S + P + H)}{S}$ , as mean ± s.d. (e) Bar diagram plot compares the CER mean chain length in FTMs and NHS. (f) Bar diagram plot compares the CER EO mean chain length in FTMs and NHS. (g) Level of unsaturation in CER subclasses NS and AS in FTMs. All data represents mean + s.d. Significance is shown for comparison between normoxia and hypoxia (with lines) and for comparison between FTMs and NHS (above NHS), otherwise indicated. Statistical differences are noted as *, ** or ***, corresponding to P < 0.05, <0.01, <0.001.