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. 2019 May 24;9:7811. doi: 10.1038/s41598-019-44204-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lipid organization in FTMs generated under hypoxia. (a) Small angle X-ray diffraction profiles of FTMs developed under normoxia and hypoxia. Orders of diffraction of the LPP are indicated by roman numbers and phase separated crystalline cholesterol is indicated by the asterisk (*). (b) Bar diagram plot of the long periodicity phase repeat distances in both FTMs. Data represent mean + s.d. Statistical testing is performed by paired t-test. (c) Representative rocking vibrations in a temperature range of 0–40 °C in FTMs developed under normoxia, hypoxia and of NHS. (d) Conformational ordering of the lipids based on the symmetric stretching peak position over a temperature range of 0–40 °C. Third order polynomial regression line connects the data points. Data represents mean ± s.e.m., n = 4.