Fig. 2.

Structure of the 2:1 Ptch1–ShhN_p complex. a Cryo-EM map for the 2:1 Ptch1–ShhN_p complex. Higher resolution was achieved after applying adapted mask to dimeric Ptch1 with one ShhN_p. Left panel: local resolution map calculated using RELION 2.0. Right panel: domain-colored map with the same color code for our previous report¹⁹. b The gold-standard Fourier shell correlation curve for the cryo-EM map. c Overall structure of one ShhN_p bound to two Ptch1. To facilitate illustration, we named the two Ptch1 protomers as Ptch1-G (G for globular) and Ptch1-P (P for palmitoylate and peptide). The N-terminal palmitoyl moiety is highlighted as violet sphere. The Ca²⁺ and Zn²⁺ ions are shown as green and purple spheres, respectively. The glycosyl moieties are shown as black sticks. d ECDs and ShhN_p viewed from extracellular view. e Transmembrane domains are shown in the extracellular view. All structure figures were prepared in PyMol⁶²