Table 1.

Primers used for subcloning and site-directed mutagenesis

Name of construct	Forward primer (5′ → 3′)	Reverse primer (5′ → 3′)		Enzyme	Nuclear localization
pDsRed2-C1-1NLS	GTC GAC GGT ACC GCG GGC AGG AAA AAG AAG CGC AAG CGC GGA TCC ACC GGA TCT		TCT AGA TCC GGT GGA TCC GCG CTT GCG CTT CTT TTT CCT GCC CGC GGT ACC GTC		+
S100A10	GGA GCT CCT CGA GCA AGCTTC ATG CCA TCT CAA ATG		GGC CAC CTA GGG CAG CTG CTT CTT TCC CTT CTG	Xho I, Sa I	_

Primer of pDsRed2-C1-1NLS contains an NLS sequence. Primer of S100A10 contains the enzyme (Xho I, Sal I) sequence and is used for subcloning. pDsRed2-C1-S100A10-1NLS is constructed using two primers in sequence