Figure 7.

Diosmetin (Dio) impairs growth of A549 cells and enhances paclitaxel efficacy in vivo. Dio (50 mg·kg⁻¹, i.p., three times a week); Dio plus NAC (7 mg·ml⁻¹ given in the drinking water for the length of the experiment); PTX (10 mg·kg⁻¹, i.p., three times a week); and Dio plus the PTX treatment inhibit tumour volume (a, b) and tumour weight (c). Histopathological analyses of major organs from control and Dio treatment group (d). (e) The tumour tissues were stained with antibody to Nrf2 and Ki67. Apoptotic nuclei with fragmented DNA were detected by TUNEL staining. Magnification, 20×. The positive staining of Nrf2 expression per field from paraffin‐embedded sections of control cells or those treated with Dio was determined by immunohistochemistry and morphometric quantification. Data shown are means ± SD; n = 5. *P < 0.05, significantly different from control group; ^# P < 0.05, significantly different as indicated. (f) Tumour tissues ROS level. Data shown are means ± SD; n = 5. *P < 0.05, significantly different from control group, ns: not significant; ^# P < 0.05, significantly different as indicated