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. 2018 Dec 26;176(12):1922–1931. doi: 10.1111/bph.14536

Figure 2.

Figure 2

Characterization of aortic leukocytes following P. gingivalis immunization and low‐dose Ang II infusion. Mice were immunized with an i.p. injection of P. gingivalis formulated with alum and IL‐12 (Alum/PG/IL‐12) and are compared with control mice that received an i.p. injection of alum only (Alum). Following immunization, both groups received a 14 day infusion of a low dose of Ang II infusion (0.25 mg·kg−1·day−1). Flow cytometry was used to quantify total leukocytes (A, CD45+), *P < 0.05 compared to control, n = 8 in each group. Within the CD45+ cells, the percentage of T cells, B cells, macrophages, NK cells and dendritic cells were quantified (B). T cells – CD3+; lymphocytes B (B cells) – CD19+; macrophages (Mf) – I‐Ab+/CD11b+; dendritic cells (DC) – I‐Ab+/CD11c+; and NK cells (NK) – NK1.1+, *P < 0.05 versus control, n = 8 (C). T‐cell infiltration in aortas of P. gingivalis‐immunized mice and controls. T‐cell subpopulations (CD4+ and CD8+ T cells were studied) by flow cytometry following a 14 day infusion of a low‐dose of Ang II, *P < 0.05 compared to control, n = 8 in each group.