Figure 4.

Effect of 1,2,3,4,6‐penta‐O‐galloyl‐β‐d‐glucose (PGG) on cytokine production by T‐cells. Freshly isolated splenocytes were stimulated with leukocyte activating cocktail and were cultured in the presence of PGG (final concentration 20 μM) for 4 hr. After this time, intracellular expressions of (a) TNF, (b) IFN‐γ, and (c) IL‐17A within (a–c) CD4⁺, (a, b) CD8⁺, and (a–c) CD3⁺CD4⁻CD8⁻ were assessed by flow cytometry. Average values + SEM and representative flow cytometric examples are shown (n = 6). *P < 0.05