Figure 5.

Effect of 1,2,3,4,6‐penta‐O‐galloyl‐β‐d‐glucose (PGG) on cytokine production by T‐cells during Ang II‐dependent hypertension. Leukocytes isolated from perivascular adipose tissue of sham‐ and Ang II‐infused mice treated with PGG or placebo were stimulated with leukocyte‐activating cocktail and were cultured for 4 hr. After this time, intracellular expressions of (a) TNF, (b) IFN‐γ, and (c) IL‐17A within (a–c) CD4⁺, (a, b) CD8⁺, and (a–c) CD3⁺CD4⁻CD8⁻ were assessed by flow cytometry. Average values + SEM and representative flow cytometric examples for Ang II‐treated with PGG or placebo are shown (n = 5 for sham and n = 6–7 for Ang II). *P < 0.05