Table 1.
Effect of PGG (20 μM) on the expression of selected receptors on T‐cells
| CD3+ | CD4+ | CD8+ | CD3+CD4−CD8− | |||||
|---|---|---|---|---|---|---|---|---|
| Placebo | PGG | Placebo | PGG | Placebo | PGG | Placebo | PGG | |
| CCR5 % (mean) | 33 ± 1.3 (328 ± 17) | 30.5 ± 0.6* (301 ± 4.4) | 32 ± 1 (352 ± 22.5) | 29 ± 1* (295 ± 4.3)* | 32.9 ± 1,3 (311 ± 18) | 30.7 ± 0.5 (295 ± 3.9) | 36.7 ± 2 (305 ± 6.4) | 35 ± 1.7 (308 ± 15) |
| CD25 % (mean) | 46.4 ± 4 (2,144 ± 66) | 45 ± 4 (1,785 ± 54)* | 55.9 ± 5.6 (2,608 ± 147) | 48.8 ± 4.4 (2,193 ± 69)* | 40.7 ± 3.76 (1,770 ± 74) | 41.5 ± 4 (1,420 ± 83)* | 47.7 ± 3.8 (1,934 ± 100) | 53 ± 3.8* (1,762 ± 57) |
| CD44 % (mean) | 75 ± 2 (5,247 ± 276) | 74 ± 1.9 (5,375 ± 270) | 93 ± 1.16 (4,176 ± 321) | 93 ± 1 (4,282 ± 238) | 60 ± 2.8 (4,384 ± 159) | 58 ± 2 (4,670 ± 289) | 87.9 ± 2.4 (13,499 ± 813) | 90 ± 1.7 (13,551 ± 814) |
Note: Freshly isolated splenocytes were cultured on anti‐CD3‐coated plates in the presence of PGG (final concentration 20 μM) for 20 hr. Expressions of CCR5, CD25, and CD44 on T‐cell (CD3+) and their subsets (CD4+, CD8+, and CD3+CD4−CD8−) cultured with the presence of PGG or placebo. The percentage (%) of the CCR5, CD25, and CD44 positive cells within T‐cell (CD3+) compartment and their subsets are indicated. Corresponding values of mean intensity of fluorescence (mean) on individual cell populations are provided (n = 7).
*
P < 0.05.