Table 1.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Characteristics | References |
|---|---|---|
| Strains | ||
| DE719 | O2:K1 | [14] |
| ΔcpxR | cpxR gene deletion mutant in DE719 | This study |
| CΔcpxR | ΔcpxR with plasmid pSTV28-cpxR | This study |
| DH5α-pRCL | DH5α with plasmid pRCL | This study |
| DH5α-pRCL-Phcp2B | DH5α with plasmid pRCL-Phcp2B | This study |
| DE719-pBAD | DE719 with plasmid pBAD/Myc-His | This study |
| DE719-pnlpE | DE719 with plasmid pBAD-nlpE | This study |
| ΔcpxR-pBAD | ΔcpxR with plasmid pBAD/Myc-His | This study |
| ΔcpxR-pnlpE | ΔcpxR with plasmid pBAD-nlpE | This study |
| DH5α | F-, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ- | TIANGEN |
| BL21 (DE3) | F-, ompT, hsdS (r−B m−B) gal, dcm (DE3) | TIANGEN |
| Plasmids | ||
| pET28a(+) | Kan, F1 origin, His tag | Novagen |
| pET28a-cpxR | pET28a(+) carrying cpxR gene | This study |
| pSTV28 | Cm, p15A origin | Takara |
| pSTV28-cpxR | pSTV28 derivative harboring cpxR gene | This study |
| pBAD/Myc-His | Amp, ColE1 derivative cloning vector, pBAD (ara) promoter | Invitrogen |
| pBAD-nlpE | pBAD/Myc-His expressing E. coli K12 NlpE-His from the pBAD (ara) promoter | This study |
| pRCL | Cm, promoterless lacZ, | This study |
| pRCL-Phcp2B | pRCL harboring hcp2B promoter | This study |
| pKD46 | Amp, expresses λ red recombinase | [45] |
| pKD3 | Cm, template plasmid | [45] |
| pCP20 | Cm, Amp, yeast Flp recombinase gene, FLP | [45] |