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. 2019 May 24;7:138. doi: 10.1186/s40425-019-0612-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Comparative analysis of the cytotoxicity and effector molecule secretion by NK-92 and NK101. a Indicated cell lines were co-cultured with NK101 (black bar) and NK-92 (white bar) at an effector-to-target ratio of 4:1 for 24 h. The percentages of apoptotic tumor cells were quantified by Annexin-V and fixable viability dye staining via flow cytometry. Percentage of specific lysis was calculated by the formula described in Online Additional file 2. *p < 0.05, **p < 0.01. b IFN-γ, TNF-α, perforin, and granzyme B concentrations in the co-culture supernatants were determined by ELISA. All data represent mean ± SD of 3 independent experiments. **p < 0.01