Fig. 4.

The treatment combining enzalutamide with USP14 inhibition arrests cell cycle progression MDA-MB453 and MCF-7 cells were treated with the indicated agents as described in Fig. 3 and then either processed for fluorescence-activated cell sorting analysis (FACS) to detect cell cycle distribution (a) and (b) or subject to total protein extraction for western blot analyses of the indicated cell cycle regulator proteins (c). GAPDH was used as a loading control. Representative images (a) and a summary of cell cycle distribution b derived from the FACS or representative images of the western blot analyses (c) are shown