Figure 2.

Median of the percentage of CD4⁺ memory T cell subsets: A) T Naïve cells (CD45RA⁺CD197⁺), B) T effector memory cells (T_EM; CD45RA^-CD197^-) and C) T central memory cells (T_CM; CD45RA^-CD197⁺) of studied participants’ blood samples during the experiment at the beginning of preparatory phase to spring and autumn competition round, respectively. CD4⁺ memory T cell subsets analysis protocol was performed using commercial antibodies assay kit (Human Naïve/Memory T Cell Panel) according to the manufacturer instructions and analysed using BD Accuri™ C6 flow cytometer. The midpoint represents median; box represents inter quartile range; whiskers represent min-max range. Significance level of differences observed between spring and autumn experiment was calculated using Wilcoxon’s matched-pairs test. Significance level of differences observed between analysed time points (pre-exercise vs. post-exercise vs. recovery) was assessed using Friedman’s analysis of variance followed by post-hoc Dunn’s test with Bonferroni correction.