. Author manuscript; available in PMC: 2019 Dec 19.

Published in final edited form as: J Am Chem Soc. 2018 Dec 5;140(50):17444–17455. doi: 10.1021/jacs.8b06354

Table 1.

List of abbreviations used for proteins.

Abbreviation	Construct ^a
CP	S100A8/S100A9
A9(ΔM1)	S100A9 lacking Met1
A9(ΔM1-M5)	S100A9 lacking Met1, Thr2, Cys3, Lys4, and Met5
A9(C3S)	S100A9(C3S)
A8+O	S100A8 with 1 additional oxygen atom
A9+nO	S100A9 with n additional oxygen atoms
CP-Ser	S100A8(C42S)/S100A9(C3S)
¹⁵N-CP-Ser	[¹⁵N]-S100A8(C42S)/[¹⁵N]-S100A9(C3S)
¹⁵N-A8(C42S)	[¹⁵N]-S100A8(C42S)
¹⁵N-A9(C3S)	[¹⁵N]-S100A9(C3S)
M63A	S100A8(C42S)/S100A9(C3S)(M63A)
M81A	S100A8(C42S)/S100A9(C3S)(M81A)
M83A	S100A8(C42S)/S100A9(C3S)(M83A)
CP-Ser O₄	CP-Ser with 4 oxidized Met residues ^b
CP-Ser O₅	CP-Ser with 5 oxidized Met residues ^b
MetO-disulfide-linked CP	CP with disulfide bonds and oxidized Met residues

All recombinant S100A9 lacked the N-terminal Met residue to due excision by methionine aminopeptidase during overexpression in E. coli.

These forms are the predominant species in the CP-Ser O₄ and CP-Ser O₅ preparations.